Anti-MBP(Thr232) (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-5474R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  MBP Thr232
• Specificity
  This antibody recognizes Thr229 in mice and Thr122 in ratMBP(Thr232)
• Modification
  Phosphorylation
• Modification site
  Thr232
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human MBP around the phosphorylation site of Thr232
• Gene ID
  4155
• Swiss Prot
  P02686
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein
• Also known as
  MBP(Thr232) Polyclonal Antibody
• Other name
  Anti-MBP(Thr232) Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  MBP   Thr232  
• Gene symbol
  MBP, PRG2, ENO1, HIVEP2, HIVEP1
• Short name
  Anti-MBP(Thr232)
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  mbp
• Alternative name
  MBP(Thr232) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6925
• Gene
  MBP
• Long gene name
  myelin basic protein
• Locus
  18q23
• Discovery year
  1986-01-01
• Entrez gene record
  4155
• Pubmed identfication
  • 2425357
• RefSeq identity
  • NM_001025081
• VEGA ID
  OTTHUMG00000132874

Gene info

• Identity
  HGNC:9362
• Gene
  PRG2
• Long gene name
  proteoglycan 2, pro eosinophil major basic protein
• Synonyms gene name
  • proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• Synonyms
  • MBP
  • BMPG
  • proMBP
• Synonyms name
  • eosinophil major basic protein
• GenBank acession
  • BC005929
• Locus
  11q12.1
• Discovery year
  1993-09-29
• Entrez gene record
  5553
• Pubmed identfication
  • 1565101
• RefSeq identity
  • NM_002728
• Classification
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000167027

Gene info

• Identity
  HGNC:3350
• Gene
  ENO1
• Long gene name
  enolase 1
• Synonyms gene
  • ENO1L1
  • MPB1
• Synonyms gene name
  • enolase 1, (alpha)
• Synonyms
  • PPH
  • MBP-1
• Synonyms name
  • alpha-enolase
• GenBank acession
  • BC022545
• Locus
  1p36.23
• Discovery year
  1989-04-21
• Entrez gene record
  2023
• Pubmed identfication
  • 9653645
  • 9691177
• RefSeq identity
  • NM_001428
• Classification
  • Enolases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000001773
• Locus Specific Databases
  • LRG_1165

Gene info

• Identity
  HGNC:4921
• Gene
  HIVEP2
• Long gene name
  HIVEP zinc finger 2
• Synonyms gene name
  • human immunodeficiency virus type I enhancer-binding protein 2
  • human immunodeficiency virus type I enhancer binding protein 2
• Synonyms
  • MBP-2
  • HIV-EP2
  • MIBP1
  • ZAS2
  • Schnurri-2
  • ZNF40B
• Synonyms name
  • c-myc intron binding protein 1
• GenBank acession
  • M60119
• Locus
  6q24.2
• Discovery year
  1992-10-21
• Entrez gene record
  3097
• Pubmed identfication
  • 1733857
  • 2022670
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000015713

Gene info

• Identity
  HGNC:4920
• Gene
  HIVEP1
• Long gene name
  HIVEP zinc finger 1
• Synonyms gene
  • ZNF40
• Synonyms gene name
  • human immunodeficiency virus type I enhancer-binding protein 1
  • zinc finger protein 40
  • human immunodeficiency virus type I enhancer binding protein 1
• Synonyms
  • CIRIP
  • MBP-1
  • CRYBP1
  • PRDII-BF1
  • ZAS1
  • Schnurri-1
  • ZNF40A
• GenBank acession
  • J05011
• Locus
  6p24.1
• Discovery year
  1990-10-04
• Entrez gene record
  3096
• Pubmed identfication
  • 2108316
  • 2037300
  • 2504707
• RefSeq identity
  • NM_002114
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000014265

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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