FISH Antibody, Biotin Conjugated
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Catalog numberbs-11645R-Biotin
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Price:
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Size0.1ml
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Long nameFISH Polyclonal Antibody, Biotin Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationBiotin
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenFISH
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SpecificityThis is a highly specific antibody against FISH.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic peptide derived from human FISH
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Tested applicationsIHC-P
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Recommended dilutionsIHC-P(1:100-500)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundFish, a potential Src substrate, is a broadly expressed adaptor protein containing five SH3 domains and a phox homology (PX) domain (1). The Src family of protein tyrosine kinases act in signal transduction pathways (2-4). Src kinases vary in expression but are strongly regulated in vivo; catalytic activity is repressed by interacting with the SH3 domain (5-7). In Src-transformed fibroblasts and in normal cells treated with certain growth factors fish is tyrosine-phosphorylated (1). Treatment of cells with cytochalasin D results in rapid tyrosine phosphorylation of fish, along with activation of Src (1). Fish is likely to be involved in tyrosine kinase signaling and may have a role in cytoskeletal changes (1).
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation EmissionN/A
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SynonymsAdapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugationBiotinylated
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French translationanticorps
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Gene target
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Gene symbolSH3PXD2A
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Short nameAnti-FISH
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelBiotin
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SpeciesFish, Fishes
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Alternative nameAnti-FISH PAb Biotin
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameSH3 and PX domains 2A
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Synonyms gene
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Synonyms gene name
- SH3 multiple domains 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
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Tree numbers
- E01.370.225.500.620.670.325.350
- E01.370.225.750.600.670.325.350
- E05.200.500.620.670.325.350
- E05.200.750.600.670.325.350
- E05.393.285.350
- E05.393.661.475.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data