EYA4 Antibody, FITC Conjugated

• Catalog number
  bs-13126R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  EYA4 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  EYA4
• Specificity
  This is a highly specific antibody against EYA4.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human EYA4
• Gene ID number
  2070
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  A gene of chromosome 6q23 encodes the 640 amino acid protein, EYA4 (eyes absent) (1). EYA is one of four members of the eyes absent family (1). A 271 amino acid domain at the carboxyl terminal is highly conserved amongst the members of the eyes absent family (1). EYA4 is expressed in the craniofacial mesenchyme, the dermamyotome, and the limb (1). The conserved region in other EYA proteins interacts with SIX, DACH, and G-proteins, which regulate transcription in early embryonic development (1,2,3,4). SIX translocates EYA1-3 to the nucleus, and G-proteins can stop this interaction (3,4). Premature stop codon mutations in EYA4 cause postlingual, progressive autosomal dominant hearing loss in humans (2). This shows that EYA4 is also vital to the mature organ of Corti (2). EYA4 may cause oculo-dento-digital syndrome, based on its expression pattern and map postion (1).
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  CMD1J; Deafness, autosomal dominant 10; DFNA 10; DFNA10; dJ78N10.1 eyes absent Drosophila homolog 4; dJ78N10.1 eyes absent; EYA 4; eya4; EYA4_HUMAN; Eyes absent 4; Eyes absent homolog 4 Drosophila; Eyes absent homolog 4; HGNC:3522; OTTHUMP00000040267.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  EYA4   Conjugated  
• Gene symbol
  TARID, EYA4
• Short name
  Anti-EYA4
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-EYA4 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:50506
• Gene
  TARID
• Long gene name
  TCF21 antisense RNA inducing promoter demethylation
• Synonyms gene
  • EYA4-AS1
• Synonyms gene name
  • EYA4 antisense RNA 1
• Locus
  6q23.2
• Discovery year
  2014-05-13
• Entrez gene record
  100507308
• Pubmed identfication
  • 25087872
• RefSeq identity
  • NR_109982
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000015607

Gene info

• Identity
  HGNC:3522
• Gene
  EYA4
• Long gene name
  EYA transcriptional coactivator and phosphatase 4
• Synonyms gene
  • DFNA10
  • CMD1J
• Synonyms gene name
  • eyes absent (Drosophila) homolog 4
  • eyes absent homolog 4 (Drosophila)
• GenBank acession
  • Y17114
• Locus
  6q23.2
• Discovery year
  1998-07-15
• Entrez gene record
  2070
• Pubmed identfication
  • 9887327
  • 11159937
• RefSeq identity
  • NM_004100
• Classification
  • EYA transcriptional coactivator and phosphatases
• VEGA ID
  OTTHUMG00000015602
• Locus Specific Databases
  • Hereditary Hearing Loss Homepage
  • LRG_418

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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