Anti-PRK1 (Thr774)/PRK2 (Thr816), ALEXA Fluor 594

• Catalog number
  GENTObs-3347R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  PRK1 Thr774 + PRK2 Thr816
• Specificity
  This antibody reacts specifically with PRK1 (Thr774)/PRK2 (Thr816)
• Modification
  Phosphorylation
• Modification site
  Thr816
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human PRK1 around the phosphorylation site of Thr774
• Gene ID
  5585
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF possesses an HIF-1 binding site; its expression is induced by hypoxia and restricted to the steroidogenic glands (ovary, testis, adrenal and placenta). Expression of EG-VEGF is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner. EG-VEGF is an example of a class of highly specific mitogens that act to regulate proliferation and differentiation of the vascular endothelium in a tissue-specific manner. It is expressed primarily in one type of tissue and acts selectively on one type of endothelium. EG-VEGF, possibly through binding to a G protein-coupled receptor, results in the activation of MAPK p44/42 and phosphatidylinositol 3-kinase signaling pathways, leading to proliferation, migration and survival of responsive endothelial cells .
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Prokineticin-1/Endocrine-gland-derived vascular endothelial growth factor; PROK1; EGVEGF; PK1; EG-VEGF; PRK1.
• Also known as
  PRK1 (Thr774)/PRK2 (Thr816) Antibody
• Other name
  Anti-PRK1 (Thr774)/PRK2 (Thr816)
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  PRK1   Thr774   PRK2   Thr816  
• Gene symbol
  PROK1, PKN2, PKN1
• Short name
  Anti-PRK1 (Thr774)/PRK2 (Thr816)
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  PRK1 (Thr774)/PRK2 (Thr816) Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:18454
• Gene
  PROK1
• Long gene name
  prokineticin 1
• Synonyms
  • PK1
  • PRK1
  • EGVEGF
• Synonyms name
  • black mamba toxin-related protein
  • mambakine
• GenBank acession
  • AF333024
• Locus
  1p13.3
• Discovery year
  2002-07-22
• Entrez gene record
  84432
• Pubmed identfication
  • 11259612
• RefSeq identity
  • NM_032414
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000011569

Gene info

• Identity
  HGNC:9406
• Gene
  PKN2
• Long gene name
  protein kinase N2
• Synonyms gene
  • PRKCL2
• Synonyms gene name
  • protein kinase C-like 2
• Synonyms
  • PRK2
  • Pak-2
  • STK7
• Synonyms name
  • cardiolipin-activated protein kinase Pak2
• GenBank acession
  • U33052
• Locus
  1p22.2
• Discovery year
  1997-10-10
• Entrez gene record
  5586
• Pubmed identfication
  • 7988719
  • 7851406
• RefSeq identity
  • NM_006256
• Classification
  • C2 domain containing protein kinases
  • AGC family kinases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000010074

Gene info

• Identity
  HGNC:9405
• Gene
  PKN1
• Long gene name
  protein kinase N1
• Synonyms gene
  • PRKCL1
• Synonyms gene name
  • protein kinase C-like 1
• Synonyms
  • DBK
  • PRK1
  • PKN
  • MGC46204
  • PAK1
• GenBank acession
  • S75546
• Locus
  19p13.12
• Discovery year
  1997-10-10
• Entrez gene record
  5585
• Pubmed identfication
  • 9570957
• RefSeq identity
  • NM_002741
  • NM_213560
• Classification
  • C2 domain containing protein kinases
  • AGC family kinases
• VEGA ID
  OTTHUMG00000039611

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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