2X PCR Pre-Mix 3 (High-Taq) without Band Sharpener (5*1ml)
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Catalog number
9K-002-0013-5000
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Price
Please ask
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Size
5X1mL
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Availability and ordering
Please contact Gentaur/Genprice team to check availability and to request a quote
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Remarks
This product is still available to order. Please contact us for more information.
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Note
This product is produced in USA according to the goods manufacturing practices and is designed specifically for Research Use Only.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
2X PCR Pre- 3 ( -Taq) without Band Sharpener (5*1ml)
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Technique
PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. biobasics TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name
2X PCR test kit Pre-Mix 3 (High-Taq) without Band Sharpener (5*1ml)
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Alternative technique
dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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