2X High-Taq PCR Premix with 1.0X Band Sharpener (5*1mL)
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Catalog number
9K-002-0012-5000
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Price
Please ask
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Size
5X1mL
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Availability and ordering
Please contact Gentaur/Genprice team to check availability and to request a quote
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Remarks
This product is still available to order. Please contact us for more information.
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Note
This product is produced in USA according to the goods manufacturing practices and is designed specifically for Research Use Only.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Description
Premixes for PCR contain dNTPs and MgCl. A PCR premix is much easier for DNA amplification than standard TAQ polymerase.
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Gene target
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Short name
2X -Taq PCR Premix with 1 0X Band Sharpener (5*1mL)
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Technique
Premix, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. premix, Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. biobasics TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name
2X High-Taq PCR test kit Premix including 1.0X Band Sharpener (5*1mL)
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Alternative technique
easyuse, dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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