Taq Mix (2x) (PCR Master Mix (2x))

  • Catalog number
  • Price
    Please ask
  • Size
    1 ml
  • Reagent properties
    Molecular biology DNA amplification enzymes and reagents
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
  • Gene target
    Taq   Master  
  • Short name
    Taq (2x) (PCR Master (2x))
  • Technique
    PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Research sys TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
  • Alternative name
    Taq Mix (2x) (PCR test kit Master Mix (2x))
  • Alternative technique
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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