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Availability
Please contact us to check the availability
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Immunogen
Pyrraline-HSA
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Species reactivity
not relevant
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Product presentation
Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace (Casein-containing solution, Dainippon Co.) and 0.1% ProClin (Rohm & Haas) as a preservative.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
H12
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Product is supplied as
liquid concentrate
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Recommended concentration for use
ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml
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Protein concentration
0.25 mg/ml
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Tested applications
IHC, ELISA
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Recommended secondary reagent
Streptavidin:HRPO conjugate (Art. No. ZU054) or alkaline phosphatase conjugate (Art.-Nr. ZU051) in combination with a suitable substrate.
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. Miyata S., and Monnier V. (1992): Immunohistochemical detection of advanced glycosylation end products in diabetic tissues using monoclonal antibody to pyrraline, J. Clin. Invest. 89(4) : 1102-1112. 3. Smith M.A., Taneda S., Richey P.L., Miyata S., Yan S.D., Stren D., Sayre L.M., Monnier V.M., Perry G. (1994): Advanced Maillard reaction end products are associated with Alzheimer disease pathology. Proc. Natl. Acad. Sci. USA91(12) : 5710-5714. 4. Odetti P., Angelini G., Dapino D., Zaccheo D., Garibaldi S., Dagna-Bricarelli F., Piombo G., Perry G., Smith M., Traverso N., Tabaton M..(1998): Early glycoxidation damage in brains from Down’s syndrome. Biochem. Biophys. Res. Commun. 243(3) : 849-851.
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus