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Availability
Please contact us to check the availability
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Immunogen
Advanced Glycation End Products-BSA
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Specificity
Positive: Advanced Glycation Endproducts, AGE-HSA, AGE-BSA, AGE-Hb, AGE-Collagen,AGE-Lys-derivatives (AGE-alpha-Tos-Lys, AGE-alpha-Tos-Lys-o-Me), AGE-monoamino carboxylic acids (AGE-beta-Alanine, AGE-gamma-aminobutyric acid, AGE-epsilon-aminocaproic acid) - negativ: early stages of the Maillard reaction (Schiff's base adducts, Amadori rearrangement products), unmodified protein HSA, BSA, Hb, Collagen, poly-Lys, Lys-derivatives (alpha-Tos-Lys, alpha-Tos-Lys-o-Me), unmodified monoamino carboxylic acids (beta-alanine, gamma-amino butyric acid, epsilon-aminocaproic acid), FFI, Pyrrole aldehyde, Pentosidine.
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Species reactivity
not relevant
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Product presentation
Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace (Casein-containing solution, Dainippon Co.) and 0.1% ProClin (Rohm & Haas) as a preservative
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
6D12
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Product is supplied as
liquid concentrate
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Recommended concentration for use
ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml
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Protein concentration
0.1 mg/ml
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Positive control
human lens, arteriosclerotic plaques
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Tested applications
IHC, ELISA
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Recommended secondary reagent
Streptavidin:HRPO conjugate (Art. No. ZU054) or alkaline phosphatase conjugate (Art.-Nr. ZU051) in combination with a suitable substrate.
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. Akari N., Ueno N., Chakrabarti B., Morino Y., and Horiuchi S. (1992) Immunochemical evidence of the presence of advanced glycation end products in human lens proteins and its positive correlation with aging. J. Biol. Chem. 267; 10211-10214. 3. Miyata T., Odo O., Inagi R., Iida Y., Araki N., Yamada N., Horiuchi S., Taniguchi N., Maeda, et al. (1993) ß2-Microglobulin modified with advanced glycation end products is a major component of haemodialysis-associated amyloidosis. J. Clin. Invest. 92; 1243-1252. 4. Kume S., Takeya M., Mori T., Araki N., Suzuki H., Horiuchi S., Kodama T., Miyauchi Y., and Takahashi K. (1995) Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta using a novel specific monoclonal antibody. Am J. Pathol. 147; 654-667. 5. Kimura T., Takamatsu J., Ikeda K., Kondo A., Miyakawa T., and Horiuchi S. (1996) Accumulation of advanced glycation end products of the Maillard reaction with age in human hippocampal neurons. Neurosci. Lett. 208; 53-56.
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Tips
*Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
