PsbI | PsbI, small subunit I of PSII (cyanobacterial)
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Catalog number
AS10 692
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Price
Please ask
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Size
200 µl
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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence Q54697
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS06 158 | PsbI | small subunit I of PSIIAlgal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 5000 - 1: 10 000 with standard ECL West pico (WB)
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Molecular weight expected аpparent
4.3 kDa
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Verified reactivity
Synechocystis sp. PCC 6803
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Possible reactivity
cyanobacteria
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
loads higher than 0.5 µg of chlorophyll per well are not recommended
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References
Dobakova et. al (2007). Role of the PsbI Protein inPhotosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.
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Scientific context
The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein
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Notes
None
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Protein number
Q54697
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TAIR number
Refer to NCBI
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Gene target
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Short name
PsbI | PsbI, subunit I of PSII (cyanobacterial)
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Alternative name
PsbI | PsbI, small functionnal sequence I on PSII (cyanobacterial)
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MeSH Data
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Name
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Concept
Scope note:
Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
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Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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