PsbI | PsbI, small subunit I of PSII (cyanobacterial)

  • Catalog number
    AS10 692
  • Price
    Please ask
  • Size
    200 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence Q54697
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 200 µl of sterile water
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    AS06 158 | PsbI | small subunit I of PSIIAlgal protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 5000 - 1: 10 000 with standard ECL West pico (WB)
  • Molecular weight expected аpparent
    4.3 kDa
  • Verified reactivity
    Synechocystis sp. PCC 6803
  • Possible reactivity
    cyanobacteria
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    loads higher than 0.5 µg of chlorophyll per well are not recommended
  • References
    Dobakova et. al (2007). Role of the PsbI Protein inPhotosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.
  • Scientific context
    The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein
  • Notes
    None
  • Protein number
    Q54697
  • TAIR number
    Refer to NCBI
  • Gene target
  • Short name
    PsbI | PsbI, subunit I of PSII (cyanobacterial)
  • Alternative name
    PsbI | PsbI, small functionnal sequence I on PSII (cyanobacterial)
MeSH Data
  • Name
  • Concept
    Scope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
  • Tree numbers
    • E05.478.566.380.830
    • E05.478.566.639.830
    • E05.601.470.380.830
    • E05.601.470.639.830
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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