KatG, catalase peroxidase (HPI), cyanobacterial
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Catalog number
AS08 374
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Price
Please ask
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Size
200 µl
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Available ordering format
Lyophilized
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Immunogen
Recombinant full length KatG protein from Synechocystis sp. PCC 6803 (accessions P73911 and sll1987) with six His-tag on the terminus.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
AS15 2991 | anti-Cat | Catalase (algal), rabbit antibodies AS09 501 | anti-Cat | Catalase (peroxisomal marker) plant, rabbit antibodies collection of antibodies to other cyanobacterial proteinsPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 3000 on 10 µg protein/lane with standard ECL (WB)
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Molecular weight expected аpparent
84.4 | 84.4 kDa for Synechocystis sp. PCC 6803
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Verified reactivity
Synechocystis sp. PCC 6803
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Possible reactivity
Synechococcus sp. including PCC 7302 and S.elongatus (PCC 6301, PCC 7942); other bacteria
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No reactivity
n.a.
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Supplementary information
not available at the moment
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References
Wenk et al. (2012). A universally conserved GTPase regulates the oxidative stress response in Escherichia coli. J Bio. Chem. Nov 8.
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Scientific context
catalase peroxidase (EC 1.11.1.7) is a bifunctional antioxidant enzyme encoded by the katG-gene. It exhibits both, catalase and peroxidase activity which provides a protection against oxidative stress by neutralising hydrogen peroxide. The enzyme is present in a number of bacterial taxa, including cyanobacteria.
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Notes
not avilable at the moment
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Protein number
P73911
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TAIR number
Refer to NCBI
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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Gene target
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Short name
KatG, catalase (HPI), cyanobacterial
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Label
peroxidase
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Alternative name
KatG, catalase peroxidase (HPI), cyanobacterial
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MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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