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Available ordering format
Lyophilized
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Immunogen
whole cyanobacterial (Anabaena PCC 7120) FtsZ protein Q3MC27 overexpressed in E.coli.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL), immunofluorescence (IF)
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Connected products
AS09 413 | anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyAS13 2651 | anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibody
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Recommended dilutions for use
1 : 2000 - 1: 5000 with alkaline phosphatase (WB), 1: 200 (IL) Immunogold-TEM, 1: 500 (IF)
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Molecular weight expected аpparent
44.5 | 50 kDa
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Verified reactivity
cyanobacteria including: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
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Possible reactivity
Arabidopsis thaliana, Oryza sativa, Prochlorococcus
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody can be used as a loading control antibody in cyanobacteria. For western blot detection image and immunolocalization results - please refer to the article below.Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).
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References
Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497. Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access. Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
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Scientific context
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
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Notes
To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody
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Protein number
Q3MC27
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TAIR number
Refer to NCBI
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Description
For cells, cell lines and tissues in culture till half confluency.
