gentaur logogentaur logo
x

PsaC, positive control/quantitation standard

  • Catalog number
    AS04 042S
  • Product name
    PsaC, positive control/quantitation standard
  • Size
    250 µl
  • Available ordering format
    Inquire
  • Immunogen
    to be determined
  • Raised in
    N/A
  • Clonality
    N/A
  • Clone
    N/A
  • Purification
    Affinity purified
  • How to reconstitute
    For reconstitution add 225 µl of milliQ water.Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of other protein standardsAS04 042 | anti-PsaC | PSI-C core subunit of photosystem I global rabbit antibodiescollection of other global antibodiescollection of antibodies to PSI proteins Plant and algal protein extraction buffer
  • Recommended dilutions for use
    Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a PsaC signal in this range.Positive control: a 2μl load per well is optimal for most chemiluminescent detection systems. This standard is ready-to-load and does not require any additions or heating.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
  • Molecular weight expected аpparent
    11.5 kDa (larger than native protein due to the addition of His-tag). In most gels PsaC migrates between 9 and 14 kDa
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Concentration: after adding 225 µl of sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
  • References
    Vandenhecke et al. (2015). Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxa. Photosynth Res. 2015 Apr 11. Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 | doi: 10.3389/fmars.2014.00068.Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
  • Scientific context
    PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.This product is a recombinant protein standard, source: Synechocystis PCC 6803.
  • Notes
    The PsaC protein standard can be used in combination with global anti-PsaC antibodies to quantitate PsaC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsaC protein.Quantitative western blot: detailed method description, video tutorial
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Hyperlink
    Hyperlink
  • Gene target
    PsaC /quantitation
  • Short name
    PsaC, positive control/quantitation standard
  • technique filter
    • Positive
    • Standard
    • Control
  • Technique
    Positive, Standard, Control, controls, standards
  • Alternative technique
    controls, standards
  • Tissue
    control
  • tissue filter
    • control