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AgriseraECL SuperBright (100 ml)

CAT: 0451-AS16 ECL-S-100Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0451-AS16 ECL-S-100Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Precautions
User InstructionStore reagents A and B in the darkness at 4-8°C.Mix equal volumes of reagent A and B (chemiluminescent substrate) in aclean containerand equilibrate to room temperature 30 minutes before use.Prepare your membrane prior addition of chemiluminescent substrate, by a wash with a buffer used in your protocol (PBS or TBS or TBST-T). This will allow to remove any background prior to substrate contact.Optimal visualization may be obtained up to 20 minutes after substrate contact. Usually, incubation for 2-5 is optimal.Remove excess substrate by filter paper.Cover blot with clear plastic wrap or sheet protector and expose either with x-ray film or CCD camera.In some cases Tween can quench the reaction.For best results clean containers and high quality water has to be used.HS code for this product is: 3822.00.0002.
References & Citations
Wieczoreket al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Fallahet al. (2018). Plasminogen activation is required for the development of radiation-induced dermatitis. Cell Death Dis. 2018 Oct 15;9(11):1051. doi: 10.1038/s41419-018-1106-8.Naranjoet al. (2021) NTRC Effects on Non-Photochemical Quenching Depends on PGR5. Antioxidants (Basel). 2021 Jun 3;10(6):900. doi: 10.3390/antiox10060900. PMID: 34204867; PMCID: PMC8229092.Ferreroet al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.
Storage Conditions
Store at 2°C to 8°C.Mixed working reagent is stable for several days at room temperature or at 4°C.Exceptional lot to lot consistency.Shelf life is 24 months when stored in the dark at 2°C to 8°C. Keep container tightly closed. Store away from heat or light.
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