1 | PEB (4x) | protein extraction buffer

CAT: 0451-AS08 300Size: 5x 2 mL (4x Stock)Dry Ice: NoHazardous: No
CAT#:0451-AS08 300Size:5x 2 mL (4x Stock)
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference) . The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication) .
HS Code
38221900
Reactivity
PEB has been tested on a wide range of species and tissues from Higher plants, Mosses, Llichens, Algae, Diatoms, Dinoflagellates, Cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western blotting, and Immunoprecipitation (IP) . Most of Agrisera commercial antibodies are tested on plant or algal samples extracted with this buffer. An example can be found here.
Applications
Protein extraction
Field of Research
Global Antibodies
Additionnal Information
Buffer components (4x) : contains ~ 40% v/v glycerol [HOCH2CH (OH) CH2OH], Tris-HCl [NH2C (CH2OH) 3 · HCl] pH 8.5, LDS [CH3 (CH2) 11OSO3Li], EDTA [ (HO2CCH2) 2NCH2CH2N (CH2CO2H) 2]It is recommended to include a protease inhibitor (not supplied with this buffer) from a freshly made stock while preparing the ready-to-use 1x PSB.PEB has been optimized for quantitative small-scale preparation of whole protein extracts from plant/algal tissue. Extraction using the procedure described below will result in maximum yield of proteins and diminish protein degradation and aggregation.Extracts may be quantified using detergent (LDS) compatible methods and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western Blotting, and immunoprecipitation.PEB has been tested on a wide range of species and tissues from higher plants, mosses, lichens, algae, diatoms, dinoflagellates, and cyanobacteria.
References & Citations
Altuntas et al. (2020) . Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144 (1) :35-48. doi: 10.1007/s11120-020-00727-w.Pérez-López et al. (2020) . Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784.Morin et al. (2019) . Morin et al. (2019) . Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom) Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80.Matsuo and Atsumi (2018) . Xylosylation of proteins by expression of human xylosyltransferase 2 in plants. J Biosci Bioeng. 2018 Sep;126 (3) :371-378. doi: 10.1016/j.jbiosc.2018.03.013.Brouwer et al. (2011) TheImpact ofLightIntensity onShade-InducedLeaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print) .Kosawang et al. (2011) Hydrogen yield from a hydrogenase in Frankia R43 at different levels of the carbon source propionate. Journal of Environmental Management, Jan 26
Storage Conditions
Stable at RT for at least 1 month; short-term storage (6 monthss) at 4°C and long term storage (1 year or more) at -20°C.
Product Datasheet
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