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Available ordering format
See included datasheet
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Immunogen
Purified keyhole limpet hemocyanin (KLH), whole molecule,
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applications
Western blot (WB), ELISA (ELISA), immunolocalization (IL)
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Connected products
AS99 001 | KLH | Keyhole limpet hemocyaninAS99 002 | anti-BSA rabbit antibodyAS02 023 | anti-BSA hen antibody
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Recommended dilutions for use
1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)
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Molecular weight expected аpparent
ca. 400 kDa/subunit
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Verified reactivity
Megathura crenulata - most commonly used carrier protein
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Possible reactivity
Megathura crenulata - most commonly used carrier protein
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.
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References
Refer to PubMed
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Scientific context
Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host-™s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
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Notes
protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here.
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Protein number
Q6KC56
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TAIR number
Refer to NCBI
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated