KLH, Keyhole limpet hemocyanin (40 µg, ALP-conjugated)
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Catalog numberAS99 001-ALP
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PricePlease ask
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Size40 µg
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Available ordering formatLiquid, conjugated to ALP
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ImmunogenPurified keyhole limpet hemocyanin (KLH), whole molecule,
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applicationsELISA (ELISA)
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Connected productsAS99 001 | KLH | Keyhole limpet hemocyaninAS99 002 | anti-BSA rabbit antibodyAS02 023 | anti-BSA hen antibody
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Recommended dilutions for use1: 5 000 (ELISA), 1: 5 000 (WB), 1: 1000 (IL)
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Molecular weight expected аpparentca. 400 kDa/subunit
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Verified reactivityMegathura crenulata - most commonly used carrier protein
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Possible reactivityMegathura crenulata - most commonly used carrier protein
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationAntibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.Optimal working dilution has to be determined by end user.
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Referencesto be added when available, antibody released in February 2016.
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Scientific contextKeyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host-™s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
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Notesprotein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here.
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Protein numberQ6KC56
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TAIR numberRefer to NCBI
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Gene target
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Gene symbolPDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
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Short nameKLH, Keyhole limpet hemocyanin (40 µg, ALP-conjugated)
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Techniqueantibody Conjugates
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Alternative nameKLH, Keyhole limpet hemocyanin (40 µg, ALP-coupled)
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Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameasparaginase and isoaspartyl peptidase 1
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Synonyms gene name
- asparaginase like 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin like 1
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Synonyms gene name
- attractin-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecretory leukocyte peptidase inhibitor
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Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- WAP four-disulfide core domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 27
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID