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Available ordering format
See included datasheet
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Immunogen
Purified keyhole limpet hemocyanin (KLH), whole molecule,
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution please add 50 µl of dest. water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB), ELISA (ELISA), immunolocalization (IL)
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Connected products
AS99 002 | anti-BSA rabbit antibodyAS02 023 | anti-BSA hen antibody
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Recommended dilutions for use
1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)
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Molecular weight expected аpparent
ca. 400 kDa/subunit
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Verified reactivity
Megathura crenulata - most commonly used carrier protein
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Possible reactivity
Megathura crenulata - most commonly used carrier protein
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.
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References
Geadkaew et al. (2014). Bi-functionality of Opisthorchis viverrini aquaporins. doi:10.1016/j.biochi.2014.11.013.Höglund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.
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Scientific context
Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host-™s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
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Notes
protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here.50 µg of anti-KLH antibody is enough for ca. 50 western blots
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Protein number
Q6KC56
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TAIR number
Refer to NCBI
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