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Category
Proteins & Peptides
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Long description
C-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors . It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells . Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, p85 subunit of PI3 kinase, SHP2 and CrkL, which links c-Kit directly to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders , while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors . Tyrosine 719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
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Antibody come from
Rabbit polyclonal Caspase-4 antibody was raised against a 15 amino acid peptide from near the center of human Caspase-4.
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Other description
Provided as solution in phosphate buffered saline with 0.02% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
c-Kit-N Terminal His Tag - Active Enzyme
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Antibody is raised in
Sf9 cells
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Antibody s suited for
Casp-4 (IN) antibody can be used for the detection of Caspase-4 by Western blot at 2 µg/ml. Depending on cell lines or tissues used, other cleavage products may be observed. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases Cell 2004; 117:561-74._x000B_2) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 converting enzyme.Science 1995; 267:2000-3._x000B_3) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224._x000B_4) Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 converting enzyme family of cysteine proteases.J. Biol. Chem. 1995; 270:15250-6.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Conjugation
histidine
