PRL-1 (2-173) N termnal GST tag - Active Enzyme

• Catalog number
  X1657E
• Price
  Please ask
• Size
  10 μg

• Category
  Proteins & Peptides
• Long description
  The product of the PRL-1 gene (alternate gene names PTP4A1 Synonyms:ÊDKFZp779M0721, hPTPCAAX1) is a protein tyrosine phosphatase also known as phosphatase of regenerating liver, Protein tyrosine phosphatase type IVA, member 1, PTPCAAX1 or Hypothetical protein DKFZp779M0721. Protein tyrosine phosphatases play important roles in the regulation of cell growth, development, and differentiation. PRL-1 is one of the most interesting immediate early growth response genes in regenerating liver. The gene encodes a novel 20-kDa nuclear protein tyrosine phosphatase. Other than the signature sequence for PTPases, PRL-1 is not homologous to either the dual specificity PTPases (cdc25 and MKP-1) or monospecific PTPases. PRL-1 is elevated throughout the major proliferative phase of liver regeneration when hepatocytes and nonparenchymal cells in the liver are rapidly proliferating. PR-1 is also expressed at high levels in other proliferating cells including tumor cell lines such as hepatomas. PRL-1-transfected cells showed altered growth characteristics, including a faster doubling time, growth to a greater saturation density, altered morphology, and evidence of anchorage-independent growth. Overexpression of human PRL-1 in epithelial cells results in tumor formation in_x000B_nude mice.
• Antibody come from
  Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Thr386 of human MEK1
• Other description
  Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol .
• Clone
  not specified
• Antigen antibody binding interaction
  PRL-1 (2-173) N termnal GST tag - Active Enzyme
• Antibody is raised in
  E.coli
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
• Antibody s suited for
  Antibody should be used at a 1:1000 dilution to provide for 10 miniblots in Western blotting and dot blots. Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity. Optimal concentration may be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Adams, J.P. and Sweatt, J.D. 'Molecular psychology: Roles for the ERK MAP kinase cascade in memory,' Annu. Rev. Pharmacol. Toxicol. 42, 135-163 (2002). _x000B__x000B_2. Ahn, N.G. 'The MAP kinase cascade. Discovery of a new signal transduction pathway,' Mol. Cell Biochem. 127-128, 201-209 (1993). _x000B__x000B_3. Ahn, N.G., et al. 'Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin,' J. Neurochem. 59, 147-156 (1992). _x000B__x000B_4. Crews, C.M., et al. 'The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product,' Science 258, 478-480 (1992). _x000B__x000B_5. Mansour, S.J., et al. 'Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.' J. Biochem. (Tokyo) 116, 304-314 (1994). _x000B__x000B_6. Park, S.H., et al. 'Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms,' Science 299, 1061-1064 (2003)._x000B__x000B_7. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463- 469._x000B__x000B_8. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046._x000B__x000B_9. Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos- phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.

• Gene target
  PRL-1   2-173   termnal   GST   tag   Active  
• Gene symbol
  PTP4A1, PRL, PTP4A2P1, PTP4A3, PTP4A2, MGST3, IGHVIII-2-1, OPRPN, MIR1-2, TRUND-NNN9-1
• Short name
  PRL-1 (2-173) N termnal GST tag - Active Enzyme
• Technique
  Enzyme, enzymes, Tags
• Isotype
  N/A
• Label
  unlabeled
• Alternative name
  PRL-1 (2-173) N termnal GST detection labelled - functionnal Enzyme
• Alternative technique
  enzymes

Gene info

• Identity
  HGNC:9634
• Gene
  PTP4A1
• Long gene name
  protein tyrosine phosphatase 4A1
• Synonyms gene name
  • protein tyrosine phosphatase type IVA, member 1
• Synonyms
  • PTPCAAX1
  • PRL-1
  • PRL1
• Synonyms name
  • phosphatase of regenerating liver 1
• GenBank acession
  • U48296
• Locus
  6q12
• Discovery year
  1998-09-02
• Entrez gene record
  7803
• Pubmed identfication
  • 9642300
• Classification
  • Protein tyrosine phosphatase 4A family
• VEGA ID
  OTTHUMG00000014949

Gene info

• Identity
  HGNC:9445
• Gene
  PRL
• Long gene name
  prolactin
• GenBank acession
  • D00411
• Locus
  6p22.3
• Discovery year
  1986-01-01
• Entrez gene record
  5617
• RefSeq identity
  • NM_000948
• Classification
  • Growth hormone family
  • Neuropeptides
• VEGA ID
  OTTHUMG00000016111

Gene info

• Identity
  HGNC:9637
• Gene
  PTP4A2P1
• Long gene name
  PTP4A2 pseudogene 1
• Synonyms gene
  • PTP4AP1
• Synonyms gene name
  • protein tyrosine phosphatase type IVA pseudogene 1
  • protein tyrosine phosphatase type IVA, member 2 pseudogene 1
• Synonyms
  • h-PRL-1
• Locus
  17q21.2
• Discovery year
  1996-03-14
• Entrez gene record
  5767
• Pubmed identfication
  • 8661118
  • 8931711
• RefSeq identity
  • NG_004085
• VEGA ID
  OTTHUMG00000180239

Gene info

• Identity
  HGNC:9636
• Gene
  PTP4A3
• Long gene name
  protein tyrosine phosphatase 4A3
• Synonyms gene name
  • protein tyrosine phosphatase type IVA, member 3
• Synonyms
  • PRL-3
  • PRL-R
  • PRL3
• Synonyms name
  • phosphatase of regenerating liver 3
• GenBank acession
  • AF041434
• Locus
  8q24.3
• Discovery year
  1999-09-09
• Entrez gene record
  11156
• RefSeq identity
  • NM_032611
• Classification
  • Protein tyrosine phosphatase 4A family
• VEGA ID
  OTTHUMG00000164490

Gene info

• Identity
  HGNC:9635
• Gene
  PTP4A2
• Long gene name
  protein tyrosine phosphatase 4A2
• Synonyms gene
  • PTP4A
• Synonyms gene name
  • protein tyrosine phosphatase type IVA, member 2
• Synonyms
  • HU-PP-1
  • PTPCAAX2
  • OV-1
  • ptp-IV1a
  • PRL-2
  • PRL2
• Synonyms name
  • phosphatase of regenerating liver 2
• GenBank acession
  • L48723
• Locus
  1p35.2
• Discovery year
  1996-03-14
• Entrez gene record
  8073
• Pubmed identfication
  • 8661118
  • 9514946
• RefSeq identity
  • NM_080391
• Classification
  • Protein tyrosine phosphatase 4A family
• VEGA ID
  OTTHUMG00000003801

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:5695
• Gene
  IGHVIII-2-1
• Long gene name
  immunoglobulin heavy variable (III)-2-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-2-1
  • immunoglobulin heavy variable (III)-2-1 pseudogene
• Synonyms
  • IGHV(III)-2-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28355
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152321

Gene info

• Identity
  HGNC:17279
• Gene
  OPRPN
• Long gene name
  opiorphin prepropeptide
• Synonyms gene
  • PROL1
• Synonyms gene name
  • proline rich 1
  • proline rich, lacrimal 1
• Synonyms
  • BPLP
  • PRL1
• GenBank acession
  • S83198
• Locus
  4q13.3
• Discovery year
  2001-12-04
• Entrez gene record
  58503
• Pubmed identfication
  • 8670737
  • 17101991
• RefSeq identity
  • NM_021225
• VEGA ID
  OTTHUMG00000160845

Gene info

• Identity
  HGNC:31500
• Gene
  MIR1-2
• Long gene name
  microRNA 1-2
• Synonyms gene
  • MIRN1-2
• Synonyms
  • hsa-mir-1-2
• Locus
  18q11.2
• Discovery year
  2004-04-23
• Entrez gene record
  406905
• RefSeq identity
  • NR_029662
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50821
• Gene
  TRUND-NNN9-1
• Long gene name
  tRNA-undetermined (NNN) 9-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 9-1
• GenBank acession
  • HG984098
• Locus
  2
• Discovery year
  2014-06-20
• Entrez gene record
  103482526
• Pubmed identfication
  • 18984615

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