Goat anti Rat IgM (FITC)
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Catalog numberMBS538680
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PricePlease ask
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Size1 mg
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Products_typeSecondary Antibody
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Products_short_name[Goat anti Rat IgM (FITC)]
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Products_name_syn[Goat anti Rat Immunoglobulin M secondary (FITC)]
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Products_gene_name[IgM]
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ReactivityTo be determined by end-user
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PurityGoat anti Rat IgM (FITC) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.
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FormLyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free. 0.01% (w/v) NaN3
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Storage_stabilityStore at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
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Tested_applicationELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Short nameGoat anti IgM (FITC)
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Techniqueanti, FITC, Goat, IgM, antibody to, Fluorescein, goats, IgMs
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgM, IgM
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LabelFITC
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SpeciesRat, Goats, Rats
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Alternative namecaprine antibody to Rat Immunoglobulin M (fluorecein)
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Alternative techniqueantibodies, caprine, fluorescine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data