HSP27 Antibody: FITC
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Catalog numberSMC-161B-FITC
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PricePlease ask
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Size200 µg
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Alternative NameHSP27 Antibody, Clone 5D12-A12: FITC
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Other nameMouse Anti-Human HSP27 Monoclonal IgG2b Kappa
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TargetHSP27
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ConjugateFITC
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CategoryAntibodies
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Product TypeMonoclonal
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Clone Number5D12-A12
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ImmunogenFull length human HSP27
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Immunogen SpeciesHuman
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Gene ID3315
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Swiss ProtP04792
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ApplicationsWB , IHC , ICC/IF , IP , ELISA
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Host SpeciesMouse
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Species ReactivityHuman
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Storage BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
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Concentration1 mg/ml
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SpecificityDetects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
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Storage Temperature-20°C
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Shipping TemperatureBlue Ice or 4°C
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PropertiesIf you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolHSPB1
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Short nameHSP27 Antibody: FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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IsotypeIgG2b Kappa
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LabelFITC
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Alternative nameHSP27 (antibody to-): fluorecein
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 1
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Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-09
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data