HSP27 Antibody (Ser15): FITC
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Catalog number
SPC-1260D-FITC
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Price
Please ask
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Size
100 µl
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Alernative name
Rabbit Anti-Human HSP27 (Ser15) Polyclonal
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Target
HSP27 (Ser15)
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Conjugate
FITC
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Research Area s
Cell Signaling , Protein Trafficking , Chaperone Proteins , Cell Signaling , Cytoskeleton , Microfilaments , Actin , Actin Assembly , Cell Signaling , Cytoskeleton , Microtubules , Cancer , Tumor Biomarkers , Cardiovascular System , Atherosclerosis , Cardiovascular System , Heart , Contractility ,Cancer , Heat Shock
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthesized unphosphorylated peptide derived from human HSP27 around the phosphorylation site of serine 15 (G-P-SP-W-D).
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Immunogen Species
Human
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Gene ID
3315
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Swiss Prot
P04792
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Applications
WB , IHC
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Host Species
Rabbit
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Species Reactivity Full Name
Human
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Storage Buffer
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Concentration
NA
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Specificity
Detects endogenous levels of total HSP27 protein.
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Storage Temperature
-20°C
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Shipping Temperature
Blue Ice or 4°C
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
HSPB1
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Short name
HSP27 Antibody (Ser15): FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
HSP27 (antibody to-) (Ser15): fluorecein
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Alternative technique
antibodies, fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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