HSP27 Antibody (pSer82): FITC

• Catalog number
  SPC-1264D-FITC
• Price
  Please ask
• Size
  100 µl

• Alernative name
  Rabbit Anti-Human HSP27 (pSer82) Polyclonal
• Target
  HSP27 (pSer82)
• Conjugate
  FITC
• Research Area s
  Cell Signaling , Protein Trafficking , Chaperone Proteins , Cell Signaling , Cytoskeleton , Microfilaments , Actin , Actin Assembly , Cell Signaling , Cytoskeleton , Microtubules , Cancer , Tumor Biomarkers , Cardiovascular System , Atherosclerosis , Cardiovascular System , Heart , Contractility ,Cancer , Heat Shock
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
• Immunogen Species
  Human
• Gene ID
  3315
• Swiss Prot
  P04792
• Applications
  WB , IHC , ICC/IF
• Host Species
  Rabbit
• Species Reactivity Full Name
  Human , Mouse , Rat
• Storage Buffer
  PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
• Concentration
  NA
• Specificity
  Detects endogenous levels of HSP27only when phosphorylated at serine 15.
• Storage Temperature
  -20°C
• Shipping Temperature
  Blue Ice or 4°C
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  HSP27   pSer82  
• Gene symbol
  HSPB1
• Short name
  HSP27 Antibody (pSer82): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  HSP27 (antibody to-) (pSer82): fluorecein
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:5246
• Gene
  HSPB1
• Long gene name
  heat shock protein family B (small) member 1
• Synonyms gene name
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
• Synonyms
  • HSP27
  • HSP28
  • Hs.76067
  • Hsp25
  • CMT2F
• GenBank acession
  • X54079
• Locus
  7q11.23
• Discovery year
  1991-07-09
• Entrez gene record
  3315
• Pubmed identfication
  • 2243808
  • 9344682
• Classification
  • Small heat shock proteins
• VEGA ID
  OTTHUMG00000023228
• Locus Specific Databases
  • Inherited Peripheral Neuropathies Mutation Database
  • LRG_248

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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