HAT (H4) Activity Fluorometric Assay Kit
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Catalog number
K336-100
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Price
Please ask
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Size
100 assays
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Description
Sensitive assay to measure HAT activity
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Summary
• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications- Measurement of HAT activity of immunoprecipitated samples - Measurement of HAT activity of purified enzyme preparations - Measurement of HAT activity in Nuclear Extracts.
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Detection Method
Fluorescence (Ex/Em = 535/587 nm)
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Applications
• Measurement of HAT activity of immunoprecipitated samples • Measurement of HAT activity of purified enzyme preparations • Measurement of HAT activity in Nuclear Extracts.
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Sample Type
• Immunoprecipitated HAT from cells and tissue • Recombinant enzyme • Nuclear Extract
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Features Benefits
• Simple, rapid & convenient • The assay can detect HAT Activity equivalent to as low as 1.5 µU of p300 in a variety of samples.
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Storage Conditions
-80°C
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Shipping Conditions
dry ice
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Shelf life
12 months
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Background
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and PicoProbeTM to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
TMPRSS11D
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Short name
HAT (H4) Activity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
HAT (H4) Activity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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