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Description
Simple and sensitive colorimetric assay for NADP dependant Isopropanol Dehydrogenase (ADH1-NADP) Activity in varied sample types.
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Summary
• Detection method- Absorbance (450 nm) • Sample Type: Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc., Animal tissues: such as liver, pancreas, small intestine, etc. Animal cells. • Applications: Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells, Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.
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Detection Method
Detection method- Absorbance (450 nm)
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Species Reactivity
Mammalian samples. Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc.
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Applications
• Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells. • Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.
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Sample Type
• Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc. • Animal tissues: such as liver, pancreas, small intestine, etc. • Animal cells.
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Features Benefits
Simple, colorimetric protocol. Detects ADH1-NADP activity as low as 0.001 mU in samples. Compatible with multiple sample types.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. BioVision’s Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
