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Active NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP)

  • Catalog number
    7605-1000
  • Product name
    Active NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP)
  • Size
    1 mg
  • Synonyms
    primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADP-aldehyde dehydrogenase; yeast alcohol dehydrogenase; alcohol dehydrogenase activity; aldehyde dehydrogenase (NADP) activity
  • Alternative names
    primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADP-aldehyde dehydrogenase; yeast alcohol dehydrogenase; alcohol dehydrogenase activity; aldehyde dehydrogenase (NADP) activity
  • Description
    A cyclic pentapeptide vascular-targeting drug.
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    TThis NADP-dependent isopropanol dehydrogenase has biological activity ≥60U/mg based on the following reaction using BioVision kit (#K698-100): Isopropanol + NADP+ Acetone + NADPH BioVision’s NADP/NADPH quantification colorimetric kit (#K347-100) can also be used to test the activity of NADP-dependent isopropanol dehydrogenase.
  • Unit Definition
    One unit is the amount of enzyme that will generate 1.0 µmole of NADPH per minute at pH 8 at 37 ̊C.
  • Molecular Weight
    ~40.9 kDa (SDS-PAGE)
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Liquid
  • Physical form description
    1 mg/mL solution in 50 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 50% glycerol
  • Background Information
    Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Datasheet
    Datasheet
  • Gene target
    Active NADP-dependent Isopropanol Dehydrogenase ADH1-NADP
    • Gene info
    • Identity:HGNC:249
    • Gene:ADH1A
    • Long gene name:alcohol dehydrogenase 1A (class I), alpha polypeptide
    • Discovery year:1986-01-01
    • Entrez gene record:124
    • Pubmed identification:3006456
    • RefSeq identity:NM_000667
    • Classification:Alcohol dehydrogenases
    • VEGA ID:OTTHUMG00000131026
  • Gene symbol
    ADH1A
  • Short name
    Active NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP)