Human HCC-1 (CCL14) Recombinant

• Background: Hemofiltrate C-C chemokine (HCC) -1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low- affinity agonist of CCR1 that is converted to a high- affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.
• Description: Recombinant HCC-1 is a disulfide-linked monomeric protein consisting of 73 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human HCC-1 (CCL14) mature chain was expressed inE. coli.
• UniProt: Q16627
• Endotoxin: < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 98% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 9 kDa
• Formulation: Lyophilized from 0.2 µm filtered solution of 40 mM NaCl, 10 mM phosphate buffer, pH 7.0.