Human HCC-2 (CCL15) Recombinant

CAT: 0421-90153-ASize: 5 µgDry Ice: NoHazardous: No

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CAT#:0421-90153-ASize:5 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

CCL15 is a human chemokine which acts predominantly on chemokine receptor CCR1 and to a lesser degree on CCR3. It is a potent chemoattractant for monocytes and lymphocytesin vitroand, after immunoprecipitation, CCL15 induces recruitment neutrophils, monocytes, and lymphocytes into mouse peritoneum. CCL15 is constitutively expressed in the gut and in the liver and was identified to circulate in human plasma. The N-terminus of CCL15 was found to be important to exert its full biological activity. HCC-2 shares significant sequence homology with CKb8 and the murine chemokines C10, CCF18/MRP-2, and macrophage inflammatory protein 1g (MIP-1g), which all contain six instead of four conserved cysteines.

Description

Recombinant HCC-2 is a disulfide-linked monomeric protein consisting of 92 amino acid residues and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human HCC-2 (CCL15) mature chain was expressed inE. coli.

UniProt

Q16663

Endotoxin

< 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Purity

≥ 98% by SDS-PAGE and HPLC

Format

Lyophilized protein

Reconstitution

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

Shipping Conditions

Gel Packs or Dry Ice

Storage Conditions

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Calculated Molecular Weight

10 kDa

Formulation

Lyophilized from 0.2 µm filtered solution in 100 mM NaCl, 20 mM phosphate buffer, pH 7.5.

UniProtKB · Q16663

C-C motif chemokine 15

CCL15_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q16663
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CCL15
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: B2RU34, E1P651, Q9UM74

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Chemotaxis

Molecular function

CytokineHeparin-binding

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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Alternative Products

Catalog	Name
Z100319	Recombinant Human HCC-2 (CCL15)
Z100315	Recombinant Human HCC-2 (CCL15)
MBS553121-01	Human HCC-2 (CCL15) Recombinant
RP01966-01	Recombinant Human CCL15/MIP-5/HCC-2 Protein
PPT-11034	Human CCL15/HCC-2/LKN-1/MIP-5 Recombinant Protein
MBS1574806-01	Recombinant Human CCL15/HCC-2/LKN-1/MIP-5, N-GST
bs-102094P-01	Recombinant Human CCL15/HCC-2/LKN-1/MIP-5 Protein, N-GST
P501314	CCL15/HCC-2/LKN-1/MIP-5 Protein (N-GST, Human)
P5887-01	Recombinant Human CCL15
4275-10	CCL14/HCC-1/HCC-3, human recombinant