PicoProbeTM Glycerol-3-Phosphate Assay Kit (Fluorometric)

• Catalog number
  K196-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive; Includes Paraoxonase Positive Control
• Summary
  • Detection method- Fluorescence (535/587 nm) • Applications: -Measurement of Glycerol-3-Phosphate in various tissues/cells -Analysis of carbohydrate and lipid metabolism and cell signaling -Drug screening
• Detection Method
  Fluorescence (Ex/Em 535/587 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Applications: -Measurement of Glycerol-3-Phosphate in various tissues/cells -Analysis of carbohydrate and lipid metabolism and cell signaling -Drug screening
• Sample Type
  • Animal tissues • Cell culture: Adherent or suspension cells
• Features Benefits
  • Simple, sensitive, and rapid. • This assay kit can detect G3P less than 20 pmol/well and can be used for a variety of sample types.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by glycerol-3-phosphate dehydrogenase from dihydroxyacetone phosphate. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis, rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle, or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis. BioVision’s PicoProbe™ Glycerol-3- Phosphate Assay Kit is a sensitive, fast and easy-to-use method of measuring G3P concentration in various biological samples. In this assay, G3P is converted into an intermediate by the G3P Enzyme Mix. The G3P developer then utilizes the intermediate to generate a fluorometric signal with PicoProbe™, which can be used to calculate the concentration of G3P in the sample. This assay kit can detect G3P as little as 20 pmol/well and can be used for a variety of sample types
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  PicoProbeTM   Glycerol-3-Phosphate   Kit   Fluorometric  
• Gene symbol
  MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC12, PIRC14
• Short name
  PicoProbeTM Glycerol-3-Phosphate Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbeTM Glycerol-3-Phosphate test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:35295
• Gene
  MIR1302-3
• Long gene name
  microRNA 1302-3
• Synonyms gene
  • MIRN1302-3
• Synonyms
  • hsa-mir-1302-3
• Locus
  2q14.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302128
• RefSeq identity
  • NR_031632
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:33675
• Gene
  MIR509-3
• Long gene name
  microRNA 509-3
• Synonyms gene
  • MIRN509-3
• Synonyms
  • hsa-mir-509-3
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126337
• RefSeq identity
  • NR_030629
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:37517
• Gene
  PIRC13
• Long gene name
  piwi-interacting RNA cluster 13
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313925
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37515
• Gene
  PIRC11
• Long gene name
  piwi-interacting RNA cluster 11
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313897
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37516
• Gene
  PIRC12
• Long gene name
  piwi-interacting RNA cluster 12
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313808
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37518
• Gene
  PIRC14
• Long gene name
  piwi-interacting RNA cluster 14
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313784
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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