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Protein A Agarose antibody Antibody Purification Reagent

Protein A Agarose antibody Antibody Purification Reagent is available 5 times from Nordc labs

X1192 | Protein A Agarose antibody Antibody Purification Reagent size: 5mL | 314.92 USD

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X1193 | Protein A Agarose antibody Antibody Purification Reagent size: 10mL | 517.97 USD

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X1191 | Protein A Agarose antibody Antibody Purification Reagent size: 1mL | 145.91 USD

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X1194 | Protein A Agarose antibody Antibody Purification Reagent size: 25mL | 941.11 USD

Catalog number X1194
Supplier nordc
Price 941.11 USD
Size 25mL
Long description This outstanding recombinant Protein A is coupled through a cysteine at the carboxy terminus creating an ether linkage to highly cross-linked agarose. It contains 6 mg of recombinant Protein A per ml of beads. The combinantion provides superior performance, binding 50 mg of human IgG per ml of beads under static conditions. Binding under flow will be less depending on the flow rate for all Protein A and Protein G products. Flow rate of 30 to 300 cm/hr are possible with this highly cross-linked agarose product under gravity flow, but it is not designed to pump under high pressure.
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction Protein A Agarose antibody Antibody Purification Reagent
Antibody's reacts with see techfile
Application Antibody Purification
Antibody's suited for Ligand Density: approximately 6 mg Protein A per ml of drained gel _x000B_Dynamic Binding Capacity: approximately 35 mg human IgG per ml of drained gel (estimated in a 0.7x5cm column at a linear flow rate of 300 cm/h under the following conditions: binding buffer: 0.1 M PBS pH 7.0, Eluting buffer 0.1 M citrate pH 3.0. 2.8 times excess IgG of the binding capacity was applied. _x000B_Bead Structure: 4% highly cross-linked agarose _x000B_Bead Size Range: 45 to 165 µm _x000B_Mean Bead Size: approximately 90 µm _x000B_Maximum Linear Flow Rate: >1300 cm/hour at 25 deg C in an XK 16/20 column, bed height 5 cm _x000B_pH Stability: Long term storage - 3 to 9; short term storage - 2 to 10 _x000B_Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 40¼ C in 3M NaSCN, 6M guanidine-HCl, 0.1M glycine (pH3.0), 70% ethanol; 10 hours at 23¼ C in 8M urea, 0.1M glycine (pH 11), 5.8M acetic acid (pH 2.0) _x000B_Physical stability: Negligible volume variation due to changes in pH or ionic strength _x000B_Sanitization: Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
Properties The purest agarose was used in the production of Protein A Agarose antibody Antibody Purification Reagent by nordc.If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation Agarose
Test Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
French translation anticorps
Gene targetProtein Antibody Reagent
Short name Protein A Agarose antibody Antibody Purification Reagent
Technique Purification, Antibody, Agarose
Alternative name Protein A molecular sieve (Antibody to) Antibody purification Reagent

X1203 | Protein A Agarose IP-Ready antibody Antibody Purification Reagent size: 1,5mL | 145.91 USD

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Category Other Reagents
Antibody come from n/a
Clone not specified
Antibody is raised in see techfile
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Storage 4ºC
Relevant references no information yet
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Isotype not specified
Label unlabeled
Alternative technique purifications
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