Protein G Agarose antibody Antibody Purification Reagent

• Catalog number
  X1196
• Price
  Please ask
• Size
  5mL

• Category
  Other Reagents
• Long description
  This outstanding recombinant Protein G has the albumin binding domain deleted providing very clean immunoglobin purifications. It is coupled to highly cross-linked agarose at 2 mg recombinant Protein G per ml beads using cyanogen bromide activation. The combination provides very superior performance binding 20 mg of human IgG per ml beads under static conditions. Binding under flow will be less depending on the flow rate for all Protein A and Protein G products. High flow rates are possible with this highly cross-linked agarose product under gravity flow, but it is not designed to pump under high pressure.
• Antibody come from
  n/a
• Other description
  Provided as a 50% aqueous suspension of Protein G in 20% ethanol solution.
• Clone
  not specified
• Antigen antibody binding interaction
  Protein G Agarose antibody Antibody Purification Reagent
• Antibody is raised in
  see techfile
• Antibody s reacts with
  IgG
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  IgG Purification
• Antibody s suited for
  LIGAND DENSITY: approximately 2 mg Protein G /mL of drained gel_x000B_DYNAMIC BINDING CAPACITY: approximately 18 mg human IgG/ml of drained gel (calculated as the amount of human IgG adsorbed to the gel before flow exceeded 1% of the absorbance of the ingoing solution at 30 cm/hr and a 0.92 mg/ml sample concentration.)_x000B_BEAD STRUCTURE: 4% highly crossed linked agarose_x000B_BEAD SIZE RANGE: 45-185 µm_x000B_MAXIMUM LINEAR FLOW RATE: >1300 cm/hr at 0.05 MPa (0.5 bar) in an XK 16/20 column, bed height 5 cm_x000B_MAXIMUM OPERATING BACK PRESSURE: 0.1 MPa (1 bar, 14 psi)_x000B_pH WORKING RANGE: 2-9 (Note: Protein G may hydrolyze at low pH)_x000B_pH STABILITY: long term storage and cleaning in place- pH of 3-9_x000B_CHEMICAL STABILITY: IgG binding capacity and recovery maintained after store for 7 days at_x000B_ 1) 37¼C in 1M acetic acid (pH 2.0), 2mM sodium phosphate, 1% sDS (pH 7.0), 6 M guanidine- HCL, 70% ethanol or_x000B_ 2) 2 hrs at room temperature in 0.1 M HCL, pH 1.0, 8 M urea, pH 10.5, 01 M Glycine-NaOH, ph 11_x000B_PHYSICAL STABILITY: Negligible volume variation due to changes in pH or ionic strength_x000B_SANITIZATION: Sanitize column using 70% ethanol_x000B__x000B_
• Storage
  4ºC
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Properties
  The purest agarose was used in the production of Protein G Agarose antibody Antibody Purification Reagent by nordc. If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Agarose
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
• French translation
  anticorps

• Gene target
  Protein   Reagent  
• Short name
  Protein G Agarose antibody Antibody Purification Reagent
• Technique
  Purification, Antibody, Agarose, agaroses, antibodies against human proteins, antibodies for, purified
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Protein G molecular sieve (antibody to-) (antibody to-) purification Reagent
• Alternative technique
  purifications, antibodies, separation

MeSH Data

• Name
  Chromatography, Agarose
• Concept
  Scope note: A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
• Tree numbers
  • E05.196.181.400.250.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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