Noggin, human recombinant
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Catalog number
4675-1000
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Price
Please ask
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Size
1 mg
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Synonyms
none
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Alternative_names
NOG
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Description
An inhibitor of TGF-β signal transduction
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Purity
≥95%
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Biological activity
Determined by its ability to inhibit 5 ng/ml of BMP-4-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED₅₀ for the effect is 0.05-0.08 µg/ml of Noggin
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Results
0.05-0.08 µg/ml
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Molecular Weight
23.1 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized with no additives
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Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1 to 1.0 mg/ml. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers
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Background Information
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 23.1 kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NOG
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Short name
Noggin, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Noggin, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
noggin
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Synonyms gene
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Synonyms gene name
- synostoses (multiple) syndrome 1
- symphalangism 1 (proximal)
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GenBank acession
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Locus
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Discovery year
1999-03-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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