Recombinant Human Noggin / NOG Protein (His tag)

• Catalog number
  PKSH031720-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  Noggin;SYM1;SYNS1
• Activity
  Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Sequence
  Met 1-Cys 232
• Fusion tag
  C-His
• Accession
  NP_005441.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  24.6 kDa
• AP Mol Mass
  30 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Noggin   NOG   Protein   His   tag  
• Gene symbol
  NOG
• Short name
  Recombinant Noggin / NOG Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Noggin / noggin Protein (histidine detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  noggin, SYM1 and SYNS1, NOG and IDBG-60160 and ENSG00000183691 and 9241, protein homodimerization activity, Extracellular, Nog and IDBG-208239 and ENSMUSG00000048616 and 18121, NOG and IDBG-647068 and ENSBTAG00000040282 and 538769

Gene info

• Identity
  HGNC:7866
• Gene
  NOG
• Long gene name
  noggin
• Synonyms gene
  • SYNS1
  • SYM1
• Synonyms gene name
  • synostoses (multiple) syndrome 1
  • symphalangism 1 (proximal)
• GenBank acession
  • U31202
• Locus
  17q22
• Discovery year
  1999-03-24
• Entrez gene record
  9241
• Pubmed identfication
  • 7666191
  • 10080184
  • 11545688
• RefSeq identity
  • NM_005450
• VEGA ID
  OTTHUMG00000151770

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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