Aminopeptidase N (APN/CD13) Activity Assay Kit (Fluorometric)
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Catalog number
K523-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive, minimum sample preparation required
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Summary
• Detection method - Fluorescence (Ex/Em = 384/502 nm) • Applications- Detection of CD13 activity in mammalian tissues, cell culture and purified enzyme
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Detection Method
Fluorometric (Ex/Em = 384/502 nm)
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Species Reactivity
Mammalian
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Applications
Detection of CD13 activity in mammalian tissues, cell culture and purified enzyme
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Sample Type
• Mammalian tissues: kidney, lung, liver • Cell culture: adherent or suspension cells • biological fluids (serum, plasma)
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Features Benefits
• Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Aminopeptidase N (APN, EC 3.4.11.2) also known as CD13 or membrane alanyl aminopeptidase is a Zn2+-dependent ectopeptidase that cleaves N-terminal neutral amino acids (preferentially alanine) from proteins and peptides. APN is a promiscuous, multifunction enzyme consisting of a short cytoplasmic domain, a single transmembrane helix and a large extracellular catalytic domain that can be cleaved to generate a soluble version of the enzyme. Aminopeptidase N expression is upregulated in many different human cancers and the enzyme is involved in metastatic tumor cell proliferation, invasion and angiogenesis. Serum levels of soluble APN are elevated in cancer patients compared with healthy controls and there is a strong inverse correlation between serum APN, tumor load and long-term prognosis in pancreatic, breast and colon cancers. Thus, in addition to being a promising pharmacological target for novel anti-neoplastic drugs, serum APN activity has been proposed as a non-invasive biomarker for the diagnosis and surveillance of breast and pancreatic cancers. BioVision’s Aminopeptidase N Activity Assay Kit enables rapid measurement of APN activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 384/502 nm). A selective APN inhibitor is provided for verification of specific activity. The assay is simple, high-throughput adaptable and can detect a minimum of 100 μU APN activity in a variety of biological samples
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
ANPEP
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Short name
Aminopeptidase N (APN/CD13) Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
Aminopeptidase N (APN/CD13) Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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