pGB CIAP-2 siRNA Vector Mix
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Catalog number
9517-20
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Price
Please ask
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Size
20 μg
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Description
pGB expression vector to supress CIAP-2 apoptotic activity in transfected cells. pGB expression vectors contain the human U6 RNA polymerase III promoter, which directs constitutive, high-level expression of short RNA transcripts in many cells. Each vector also contains the neomycin/kanamycin-resistance gene to provide kanamycin resistance in bacteria and the G418 resistance in mammalian cells. In addition, pGB Cloning vector which is used to clone your own insert and pGB Negative Control vector which contains an insert that does not have significant homology to mammalian genes expressed in human, mouse, and rat, and therefore can be used as a negative control for pGB-expression vectors. The pGB siRNA vectors are designed to suppress the expression of some of the most important apoptosis genes, individually. The mix of four siRNA vectors for each gene has been proven more efficient for gene suppression.
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Product Highlights
• GeneBlocker™ cIAP-2 siRNA Vector Mix • Application- The pGB siRNA vector Mix (1 µg/µl) can be transfected into mammalian cells using Lipofectamine (Invitrogen). For transient transfection, cells can be analyzed in 24-96 hours following transfections, by Western blot analysis or other detection means. For stable transfections, cells can be selected in G418 selection medium to obtain stable cell lines with the specific gene blocked. • Features and Positions: Human U6 Promoter: 1-256 Multiple cloning Site: 259-285 3’ Primer: 398-426 (GAAGCATTTATCAGGGTTATTGTCTCATG) SV40 Promoter: 470-808 Neomycin/Kanamycin Resistance ORF: 843-1634 5’ Primer: 2789-2813 (CGTCGATTTTTGTGATGCTCGTCAG) pUC Origin of Replication: 2222-3003
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Gene target
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Gene symbol
BIRC3, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2
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Short name
pGB CIAP-2 siRNA Vector
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Technique
sirna, Vectors
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Alternative name
pGB CIAP-2 small interfearing RNA integrating Desoxyribonucleic acid sequence Mix
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Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 7-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 329-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2005-07-11
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 512-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 521-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2005-07-15
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 509-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
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Identity
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Gene
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Long gene name
RNA, U6 small nuclear 2
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Synonyms
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Locus
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Discovery year
2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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