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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Purified Rat IgG (H&L)
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Raised in
Donkey
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Specificity
This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
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Cross Reactivity
No reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, Goat, Guinea Pig, hamster, horse, human, mouse, rabbit or sheep.
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Conjugate Tag
Alkaline Phosphatase
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Grade Purity
Grater than 95% pure
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Method of Purification
Affinity purified using solid phase Rat IgG (H&L)
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Source
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.50 mg/ml (lot specific)
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Form Buffer
Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Usage Recommendations
1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin name
Equus africanus asinus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
anti IgG H + L Alk Phos
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Technique
anti, IgG, antibody to, IgGs
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Host
Rat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgG, IgG
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Species
Rat, Donkeys, Rats
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Alternative name
Donkey anti Rat IgG (H + L) secondary antibody (Alk Phos)
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Alternative technique
antibodies, immunoglobulins
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Alternative to gene target
anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
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