Rabbit anti Rat IgG Alk Phos
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Catalog number
43C-CB1256
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rabbit anti-rat IgG (Alk Phos) was raised in rabbit using rat IgG F(ab')2 fragment ..
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Raised in
Rabbit
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Specificity
Rat IgG
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
Alkaline Phosphatase
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Method of Purification
Rabbit anti Rat IgG (Alk Phos) was purified by affinity chromatography.
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Concentration
Batch dependent - please inquire should you have specific requirements
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Form Buffer
0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl,, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C. DO NOT FREEZE.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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Gene target
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Short name
Rabbit anti IgG Alk Phos
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Technique
Rabbit, anti, IgG, antibody to, IgGs
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Species
Rat, Rats
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Alternative name
Rabbit anti Rat IgG secondary antibody (Alk Phos)
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Alternative technique
rabbit-anti, antibodies, immunoglobulins
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Alternative to gene target
anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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