Donkey anti Mouse IgG H + L Alk Phos
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Catalog number43R-ID003alk
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Price:
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Size500 µl
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenDonkey anti-mouse IgG (H + L) (Alk Phos) was raised in donkey using mouse IgG (H&L) ..
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Raised inDonkey
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SpecificityMurine
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Cross ReactivityShould be determined by the end user
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Conjugate TagAlkaline Phosphatase
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Method of PurificationDonkey anti Mouse IgG (H + L) (Alk Phos) was purified by affinity chromatography.
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Form BufferLyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
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StorageStore at 4 deg C.
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Shipping conditionsBlue Ice
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Tested forELISA; WB
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Usage RecommendationsELISA: 1:5,000-1:50,000, WB: 1:5,000-1:50,000
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Latin nameEquus africanus asinus, Mus musculus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short nameanti Mouse IgG H + L Alk Phos
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Techniqueanti mouse, Mouse, anti, IgG, antibody to, anti mouse antibodies, IgGs, mouses
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Hostmouse, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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IsotypeIgG, IgG
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SpeciesMouse, Donkeys, Mouses
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Alternative nameDonkey anti Mouse IgG (H + L) secondary antibody (Alk Phos)
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Alternative techniquemurine, antibodies, immunoglobulins
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Alternative to gene targetanaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data