SHEEP ANTI BOVINE IgG:Biotin
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Catalog number
MBS224185
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Price
Please ask
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Size
1 mg
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Products_type
Antibody
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Products_short_name
[IgG]
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Products_gene_name
[IgG]
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Clonality
Polyclonal
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Form
Biotin Purified IgG fraction conjugated to Biotin - liquid
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Concentration
IgG concentration 1.0 mg/ml
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Storage_stability
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.
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Tested_application
ELISA (EIA), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Short name
ANTI IgG:
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Technique
anti, IgG, antibody to, IgGs
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Host
Sheep
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Isotype
IgG, IgG
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Label
Biotin
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Species
Bovine, Bos Taurus genes are available form the Bovine Genome database. The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
ovine antibody to BOVINE Immunoglobulin G:biotinilated
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Alternative technique
antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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