Rabbit anti Sheep IgG biotin
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Catalog number
43C-CB1347
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Price
Please ask
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Size
500 µg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rabbit anti-sheep IgG (biotin) was raised in rabbit using sheep IgG F(ab')2 fragment ..
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Raised in
Rabbit
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Specificity
Sheep IgG
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
Biotin
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Method of Purification
Rabbit anti Sheep IgG (biotin) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Short name
Rabbit anti IgG
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Technique
Rabbit, anti, IgG, antibody to, IgGs
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Label
Biotin
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Rabbit anti Sheep IgG secondary antibody (biotin)
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Alternative technique
rabbit-anti, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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