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Category
Primary Antibodies
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Long description
Focal Adhesion Kinase (FAK) is a 120 kDa tyrosine kinase which become phosphorylated in response to oncogene- and extracellular-matrix integrin-dependent stimulation. Chicken, mouse and human FAK has been isolated and the deduced amino acid sequences showed identity of over 90%. It is expressed in embryonic stem cells, and ubiquitously throughout development, in all adult tissues examined and in many cell lines. The highest expression of FAK is seen in the brain, testes and in osteoclasts. It is distinct from other non-receptor protein tyrosine kinases in that its catalytic domain is flanked by amino- and carboxy-terminal domains of about 400 amino acids each. Also, it does not contain the Src homology 2 or 3 domains, which mediate protein-protein interaction, nor a myristylation site that anchors proteins to the membrane.
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Antibody come from
Bacterially expressed fusion protein consisting of the C-terminal 150 amino acids of the mouse FAK
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Focal Adhesion Kinase (FAK) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation
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Antibody s suited for
Detect focal adhesion kinase by IP/Western analysis in BALB/c 3T3 fibroblasts1. Detects a 120 kDa band. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Hanks, S.K., et al. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 1992, 89, 8487-8491_x000B__x000B_2. Ilic, D., et al. Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 1997, 110, 401-407
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Rabbit anti Focal Adhesion Kinase (FAK). This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
