CD28 FITC Monoclonal Antibody (Clone CD28.2)

• Catalog number
  6957-25
• Price
  Please ask
• Size
  25 tests

• Antibody Target
  CD28
• Target Alternative Name
  CD28
• Antibody Type
  Monoclonal
• Clone
  CD28.2
• Species Reactivities
  Human
• Immunogen Sequence
  Human CD28
• Accession
  P10747
• Gene Id
  940
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
• Purification
  Affinity chromatography.
• Application
  Flow cytometry
• Positive Control
  FACS: Human peripheral blood lymphocytes
• Application And Usages
  Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.5 µg).
• Description
  CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some sub regions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.
• Storage Condition
  4°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD28   Monoclonal   CD28  
• Gene symbol
  CD28
• Short name
  CD28 FITC Monoclonal Antibody (Clone CD28 2)
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
• Isotype
  Mouse IgG1, Kappa
• Label
  FITC
• Alternative name
  CD28 molecule fluorecein monoclonal (antibody to-) (clonality CD28 molecule.2)
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
• Clone name
  CD28

Gene info

• Identity
  HGNC:1653
• Gene
  CD28
• Long gene name
  CD28 molecule
• Synonyms gene name
  • CD28 antigen (Tp44)
• Synonyms name
  • T-cell-specific surface glycoprotein
• GenBank acession
  • J02988
• Locus
  2q33.2
• Discovery year
  1988-05-11
• Entrez gene record
  940
• Pubmed identfication
  • 1355979
• RefSeq identity
  • NM_006139
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • V-set domain containing
• VEGA ID
  OTTHUMG00000132878

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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