CD28 FITC Monoclonal Antibody (Clone CD28.2)
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Catalog number
AMM00025G
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Price
Please ask
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Size
50 ug
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Product_type
Monoclonal Antibody
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Clone
CD28.2
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Application
FC
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Storage
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Note
For research use only.
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Properties
If you buy Antibodies supplied by Leading Biology they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Leading Biology Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Leading Biology FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
CD28
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Short name
CD28 FITC Monoclonal Antibody (Clone CD28 2)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
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Host
Mouse
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Isotype
Mouse IgG1, Kappa
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Label
FITC
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Alternative name
CD28 molecule fluorecein monoclonal (antibody to-) (clonality CD28 molecule.2)
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
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Clone name
CD28
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Gene info
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Identity
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Gene
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Long gene name
CD28 molecule
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Synonyms gene name
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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