Mouse Anti-Human CD28 Monoclonal Antibody, FITC Conjugated Clone B-T3
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Catalog numberMBS335353
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PricePlease ask
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Size5x100 Tests
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Products_typeAntibody
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Products_short_name[CD28]
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Products_name_syn[CD28]
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Other_names[CD28, partial; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD_antigen: CD28]
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Products_gene_name[CD28]
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Other_gene_names[Cd28; Cd28]
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ClonalityMonoclonal
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Clone[B-T3]
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ReactivityHuman
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SpecificityRecognises the B7/BB1 receptor (B7/BB1 R), a 44 kDa protein
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PurityIon exchange chromatography
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FormPhosphate-buffered saline with 5 % BSA and 0.1 % sodium azide
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Storage_stabilityStore at +2-8 degree C for 12 months. For longer storage, freeze aliquots.
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Tested_applicationFlow Cytometry (FC/FACS)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestMyBioSource supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD28, HBA1, B4GALT3, B3GNT3, GALNT3, SNRPB, ORM2, B4GALNT3, SLC25A5, ERAL1
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Short nameMouse Anti- CD28 Monoclonal Antibody, FITC Conjugated Clone B-T3
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Techniqueanti-Human, Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, antibody Conjugates, Fluorescein, Monoclonals or monoclonal antibodies, mouses
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HostMouse
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IsotypeIgG2a
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman CD28 molecule monoclonal (antibody to-), fluorecein coupled clonality B-T3
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
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Clone nameB-T3
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Gene info
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Identity
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Gene
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Long gene nameCD28 molecule
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Synonyms gene name
- CD28 antigen (Tp44)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehemoglobin subunit alpha 1
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Synonyms gene name
- hemoglobin, alpha 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hemoglobin subunits
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-galactosyltransferase 3
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 3
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
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Synonyms
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Synonyms name
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein polypeptides B and B1
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Synonyms gene
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Synonyms
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Locus
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Discovery year1988-11-28
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Entrez gene record
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Pubmed identfication
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Classification
- Small nucleolar RNA protein coding host genes
- Sm spliceosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameorosomucoid 2
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-03-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lipocalins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-N-acetyl-galactosaminyltransferase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 25 member 5
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Synonyms gene
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Synonyms gene name
- solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-08-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameEra like 12S mitochondrial rRNA chaperone 1
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Synonyms gene name
- Era (E. coli G-protein homolog)-like 1
- Era G-protein-like 1 (E. coli)
- Era-like 12S mitochondrial rRNA chaperone 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-19
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data