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Alternative name1
Anti-RABBIT F(ab')2 ANTI RAT IgG:HRP
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Alternative name2
RABBIT F(ab')2 ANTI RAT IgG:HRP
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Alternative name3
Lapin F(ab')2 ANTI Rattus norvegicus IgG:HRP
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Alternative name4
IgG
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Alternative name5
N/A
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Other names
Ig gamma-2A chain C region; Ig gamma-2A chain C region; N/A; N/A; N/A
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Gene name
IgG
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Gene name synonims
N/A
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Other gene names
LOC679045; Igg-2a; N/A; N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Rat
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Specificity and cross reactivity
IgG Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method. Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
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Purification method
N/A
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Form Appearance
HRP (Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid)
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Concentration
IgG concentration 1.0 mg/ml
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Storage and shipping
Store at +Store productone at +4 degrees Celsius.. DO NOT FREEZE. productone should be stored undiluted. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.
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Tested for
Immunohistology - Resin (IHC), Immunohistology - Frozen (IHC), Immunohistology - Paraffin (IHC), ELISA (EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Group
f(ab')2s
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Short name
F(ab')2 Anti- IgG: Antibody
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Technique
Antibody, F(ab')2, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
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Host
Rat
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Label
HRP
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Species
Rat, Rats
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Alternative name
F(Antibody')2 Antibody toRAT Immunoglobulin G:horseradish peroxidase (antibody to-)
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Alternative technique
antibodies, secondairy, immunoglobulins
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