Chicken anti Rat IgG H + L HRP

  • Catalog number
    43R-1513
  • Price
    Please ask
  • Size
    500 µg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Rat IgG (H&L)
  • Raised in
    Chicken
  • Specificity
    This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or rabbit or serum proteins from human or rabbit.
  • Conjugate Tag
    HRP
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Rat IgG (H&L)
  • Source
    Ig fraction was prepared using eggs obtained from healthy hens of US origin.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 0.55 ml of deionized water. Prepare fresh working dilution daily.
  • Storage
    Store lyophilized material at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    Please refer to the datasheet
  • Usage Recommendations
    1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
  • Conjugation
    HRP Conjugated
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    -
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    anti IgG H + L
  • Technique
    anti, IgG, antibody to, IgGs
  • Host
    Rat
  • Isotype
    IgG, IgG
  • Label
    HRP
  • Species
    Rat, Chickens, Rats
  • Alternative name
    Chicken anti Rat IgG (H + L) secondary antibody (HRP)
  • Alternative technique
    antibodies, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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