Goat anti Rat IgG Fab'2 HRP

  • Catalog number
    43C-CJ0315
  • Price
    Please ask
  • Size
    500 µg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Goat anti-rat IgG (Fab'2) (HRP) was raised in goat using Rat IgG F(c) whole molecule ..
  • Raised in
    Goat
  • Specificity
    Rat IgG (Fab2)
  • Cross Reactivity
    Should be determined by the end user
  • Conjugate Tag
    HRP
  • Method of Purification
    Goat anti Rat IgG (Fab'2) (HRP) was purified by affinity chromatography.
  • Form Buffer
    Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3 I mMunoglobulin and protease free.
  • Storage
    Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; IHC; WB
  • Usage Recommendations
    ELISA: 1:120,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:10,000
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
  • Latin name
    Capra aegagrus hircus, Rattus norvegicus
  • Conjugation
    HRP Conjugated
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Gene target
    -
  • Gene symbol
    MIR514A2, MIR1289-2, MIR521-2
  • Short name
    Goat anti IgG Fab'2
  • Technique
    anti, Goat, Fab, IgG, antibody to, fabs, goats, IgGs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    fab'2, IgG,  Fab fragment, IgG
  • Label
    HRP
  • Species
    Rat, Goats, Rats
  • Alternative name
    Goat anti Rat IgG secondary antibody (Fab'2) (HRP)
  • Alternative technique
    antibodies, caprine, seconairy, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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