pECMV-Gli3-m-FLAG Plasmid

• Catalog number
  PVT15444
• Price
  396.42 USD
• Size
  2 ug

• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

• Gene target
  pECMV-Gli3-m  
• Short name
  pECMV-Gli3-m- Plasmid
• Technique
  plasmid, plasmids in 1
• Label
  Flag
• Alternative name
  pECMV-GLI family zinc finger 3-m-FLAG Plasmid
• Alternative technique
  plasmids
• Alternative to gene target
  GLI family zinc finger 3, ACLS and GCPS and GLI3-190 and GLI3FL and PAP-A and PAPA and PAPA1 and PAPB and PHS and PPDIV, GLI3 and IDBG-13536 and ENSG00000106571 and 2737, metal ion binding, nuclei, Gli3 and IDBG-131686 and ENSMUSG00000021318 and 14634, GLI3 and IDBG-634502 and ENSBTAG00000010671 and 785371

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