pECMV-FLAG-MDM2

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Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
Conjugation

Flag

Quantity:

Identity

HGNC:6973
Gene

MDM2
Long gene name

MDM2 proto-oncogene
Synonyms gene name
- mouse double minute 2, human homolog of; p53-binding protein
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
- Mdm2 p53 binding protein homolog (mouse)
- MDM2 proto-oncogene, E3 ubiquitin protein ligase
Synonyms
- HDM2
- MGC5370
Locus

12q15
Discovery year

1993-12-10
Entrez gene record

4193
Pubmed identfication
RefSeq identity
- NM_002392
Classification
- Zinc fingers RANBP2-type
- Ring finger proteins
VEGA ID

OTTHUMG00000142827